The TransMission is the transfection blog delivered by Mirus Bio.
Today’s TransMission is a SNiP of one of our favorite papers featuring Label IT®. Nasri and Mir et al. describe an elegant use for Label IT® in enriching CRISPR/Cas-edited cells. For use in the clinic, it is highly desirable to limit the number of nonedited cells that may compete with the therapeutic, modified cells. Read on to learn how Label IT® was used to enhance the CRISPR genome-editing workflow!
Earlier this year, Mirus Bio added RevIT™ AAV Enhancer to its established portfolio of reagents and kits for viral vector production to meet calls for more virus per transfection for any AAV construct.
Read on to learn how RevIT™ AAV Enhancer enables using less plasmid DNA to achieve high titers and percent full capsids!
Let’s ring in the new year with a list of all current FDA approved cell and gene therapies to date!
Read on to learn more about the 34 different treatments and cures.
In today’s TransMission, we answer the FAQ: “How can I increase my viral titers and improve the percentage of full AAVs?”
Today’s SNiP reminds us that we are never truly alone. It’s been estimated that over one trillion microbial cells inhabit the human body. In this study, Wojciech et al. implicate a eukaryotic gut-dwelling microbe, Blastocystis subtype 7, in host T cell dysregulation, providing a mechanism for how this microbe can cause inflammation in the gut.
Using the CHOgro® High Yield Expression System, exceptional yields of recombinant protein can be obtained by transient transfection of suspension CHO cells. Though transgene expression ceases for the vast majority of cells within one to two weeks post-transfection, a small fraction of transfected CHO cells continues to stably express the recombinant protein through random chromosomal integration of the transfected gene. In this Tip from the Bench, we share a protocol for generating stable cell lines through selection and expansion of CHO cells which produce high levels of recombinant protein.
“Shock me like an electric eel.” To creatures sharing an ecosystem in the Amazon, co-existing with electric eels and their jolting aura is a lifelong experience. A group of researchers from Nagoya University explored whether shocks from electric eels could mediate nucleic acid delivery to zebrafish larvae! Could these eels be the living embodiment of the Ingenio® EZporator® Electroporation System?
Suspension CHO cells have emerged as one of the most important cell lines for protein expression in biomanufacturing for their capacity to grow to high cell density in serum-free medium. Successful cell growth of these popular cells is dependent on several factors, which include media composition, culture format and appropriate cell maintenance. In today’s Tip from the Bench, we share a few tips for keeping your suspension CHO cells in transfection-ready shape!
Today’s TransMission explores an unconventional application of Mirus Bio’s Label IT® Nucleic Acid Labeling kits in engineering a new method of vaccine delivery, the microneedle array (MNA). MNAs are patches of micron-scale needles that delicately penetrate the skin in a painless manner, accessing an immune-rich population of cells. In this study, the authors fluorescently labeled immune-stimulating oligos using Label IT® Cy®3 and Label IT® Cy®5 to visualize their distribution on MNAs.
Read on to learn more about this unique method of stimulation!
Ideal labeling density will depend on the nucleic acid being labeled and the intended downstream application of the labeled nucleic acid. With Label IT® nucleic acid labeling technology, the labeling density can easily be adjusted. In this Tip from the Bench, we discuss two methods for tweaking the labeling density.